International Journal of Hematology and Oncology
2024, Vol 34, Num 2 Page(s): 093-101
| Back | Table of Contents | PDF | Mail to Author | |
The Effects of Pentoxifylline Administration on TGF-β1 Expression in Rats with Radiation-Induced Muscle Fibrosis
Gunay GASIMLI1,2, Merve DEMIR1,2, Baris ERTUGRUL3, Bedia CAKMAKOGLU3, Hatice BILGE BECERIR4, Merva SOLUK TEKKESIN5, Musa ALTUN6, Kivanc Bektas KAYHAN1
1Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery
2Istanbul University, Institute of Graduate Studies in Health Sciences, Department of Oral and Maxillofacial Surgery
3Istanbul University, Institute of Experimental Medical Research, Department of Molecular Medicine
4Istanbul University Oncology Institute, Department of Medical Physics
5Istanbul University Oncology Institute, Department of Tumor Pathology
6Istanbul University Oncology Institute, Department of Radiation Oncology
Keywords: Pentoxifylline, Fibrosis, Radiation therapy, TGF-β1 expression
The aim of this study was to investigate the potential influence of pentoxifylline (PTX) administration on transforming growth factor beta 1 (TGF-β1) expression and the development of radiation-induced fibrosis (RIF) in Sprague-Dawley rats. Seventeen rats (n= 17) were randomly divided into four groups: The first one received a single dose equivalent to 90 Gy of radiotherapy (RT) to the rectus femoris muscle, the second received PTX administration (25 mg/kg/day) via gavage in addition to irradiation (RT+PTX), the third group received only PTX, and the fourth group served as the controls. At the end of 24 days, fibrosis formation was assessed by light microscopy. The TGF-β1serum and tissue levels were assessed with enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction methods. Immunohistochemical staining revealed focal punctate cytoplasmic TGF-β1 staining in the irradiated group compared with the control group. Morphological changes, including increased oedema and mild collagen thickening, were observed in both the RT and RT+PTX groups. Reverse transcription polymerase chain reaction results revealed no significant differences between the RT, PT+PTX, PTX, and Control group. ELISA results showed substantial differences between the RT, RT+PTX, PTX, and Control group (p= 0.125). The Control group without therapy exhibited higher ELISA results. PTX administration did not demonstrate a positive effect on the serum and muscle expression of TGF-β1 in rats with fibrosis induced by a single dose of radiation. Additionally, TGF-β1 may not be a reliable marker of fibrosis that can be routinely used in the early stages of similar radiotherapy-related tissue damage.
Gunay GASIMLI1,2, Merve DEMIR1,2, Baris ERTUGRUL3, Bedia CAKMAKOGLU3, Hatice BILGE BECERIR4, Merva SOLUK TEKKESIN5, Musa ALTUN6, Kivanc Bektas KAYHAN1
1Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery
2Istanbul University, Institute of Graduate Studies in Health Sciences, Department of Oral and Maxillofacial Surgery
3Istanbul University, Institute of Experimental Medical Research, Department of Molecular Medicine
4Istanbul University Oncology Institute, Department of Medical Physics
5Istanbul University Oncology Institute, Department of Tumor Pathology
6Istanbul University Oncology Institute, Department of Radiation Oncology
Keywords: Pentoxifylline, Fibrosis, Radiation therapy, TGF-β1 expression
The aim of this study was to investigate the potential influence of pentoxifylline (PTX) administration on transforming growth factor beta 1 (TGF-β1) expression and the development of radiation-induced fibrosis (RIF) in Sprague-Dawley rats. Seventeen rats (n= 17) were randomly divided into four groups: The first one received a single dose equivalent to 90 Gy of radiotherapy (RT) to the rectus femoris muscle, the second received PTX administration (25 mg/kg/day) via gavage in addition to irradiation (RT+PTX), the third group received only PTX, and the fourth group served as the controls. At the end of 24 days, fibrosis formation was assessed by light microscopy. The TGF-β1serum and tissue levels were assessed with enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction methods. Immunohistochemical staining revealed focal punctate cytoplasmic TGF-β1 staining in the irradiated group compared with the control group. Morphological changes, including increased oedema and mild collagen thickening, were observed in both the RT and RT+PTX groups. Reverse transcription polymerase chain reaction results revealed no significant differences between the RT, PT+PTX, PTX, and Control group. ELISA results showed substantial differences between the RT, RT+PTX, PTX, and Control group (p= 0.125). The Control group without therapy exhibited higher ELISA results. PTX administration did not demonstrate a positive effect on the serum and muscle expression of TGF-β1 in rats with fibrosis induced by a single dose of radiation. Additionally, TGF-β1 may not be a reliable marker of fibrosis that can be routinely used in the early stages of similar radiotherapy-related tissue damage.
| Back | Table of Contents | PDF | Mail to Author | |