International Journal of Hematology and Oncology 2022, Vol 32, Num 3 Page(s): 199-204
The Importance of Tyrosine Kinase-Like Orphan Receptor 1 (ROR-1) in Chronic Lymphocytic Leukemia

Hacer Berna Afacan OZTURK1, Murat ALBAYRAK1, Abdulkerim YILDIZ1, Senem MARAL1, Pinar AKYOL1, Merih Reis ARAS1, Mesude FALAY2

1University of Health Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Department of Hematology, Ankara, TURKEY
2Duzen Laboratory Groups, Department of Biochemistry, Ankara, TURKEY

Keywords: Chronic lymphocytic leukemia, ROR-1, Flow cytometry; Prognosis
Type 1 tyrosine kinase–like orphan receptor (ROR-1) is expressed on neoplastic B cell surface in chronic lymphocytic leukemia (CLL). In this study, we aimed to determine the prognostic significance of ROR-1 expression with relationship of clinical features and therapy requirements. Retrospectively, we analyzed the medical records of CLL patients who diagnosed at Hematology department between
December 2016 and October 2019. The following panel 8 color combinations of monoclonal antibodies. While matutes scoring was accepted to indicate cell surface expression of 30% or greater, we practiced to determine its cut-off value with a ROC (receiver operating characteristic) curve. The optimal ROR-1 cut-off was evaluated 80.5%. White blood cell count, lymphocyte count and platelet count were found significantly higher in patients with ROR-1 level > 80.5% at diagnosis (p< 0.05). Furthermore, the majority of patients with ROR-1 level > 80.5% were presented with splenomegaly initially. Rates of therapy requirements was found similar in two groups. Higher higher level and uniform expression of ROR-1 may be used as a diagnostic marker rather than predictive or prognostic marker in CLL patients. Therefore, we suggest that ROR1 adding in routine antibody panels may be useful at diagnosis of CLL.