International Journal of Hematology and Oncology 2020, Vol 30, Num 2 Page(s): 036-044
Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues

Gulcin G. SIMSEK1, Serpil OGUZTUZUN2, Busra BOZER4, Murat KILIC3, Arzu K. KOCDOGAN5, Pınar KAYGIN2, Nurdan GURBUZ2, Hakan BULUS6

1University of Health Sciences, Kecioren Training and Research Hospital, Department of Pathology, Ankara, TURKEY
2Kirikkale University, Faculty of Arts and Sciences, Department of Biology, Kirikkale, TURKEY
3Ankara University Vocational School of Health Services Department of Pharmacy, Ankara, TURKEY
4Hitit University, Scientific Technical Research and Application Center, Corum, TURKEY
5Gelisim University, Health Services Vocational High School Pathology Laboratory Techniques Department, İstanbul, TURKEY
6University of Health Sciences, Kecioren Training and Research Hospital, Department of General Surgery, Ankara, TURKEY

Keywords: Gastric adenocarcinoma, Cytochrome P450 enzymes, Glutathione-S-transferase
In this study we investigated the immunohistochemical staining characteristics of cytochrome P450 1A1 (CYP1A1), CYPB1, CYP2E1 and glutathione S-transferase P1 (GSTP1), GSTT1, GSTO1, GSTK1 isoenzymes in gastric tumor and surrounding tumor free (normal) gastric tissues from 40 patients. For immunohistochemical studies, tissues were obtained from 40 patients with gastric adenocarcinoma. Tumor and non-tumoral control tissues of patients were compared according to their staining intensity. Relationships between CYP and GST isoenzyme expressions in adenocarcinoma tissues were examined by the Mann Whitney-U test, and the clinicopathological data were examined by the Spearman’s Rank Correlation test. CYP1B1, GSTT1, GSTO1 and GSTK1 expressions in gastric cancer cells were significantly higher than those in gastric normal epithelial cells (p< 0.05). However, CYP1A1, CYP2E1 and GSTP1 expressions were not significantly higher in tumor epithelium than those in normal epithelium in human gastric adenocancer (p< 0.05). Among the studied CYPs and GSTs, there was not statistically significant association between the studied isoenzyme expressions and age, gender and tumor grade (p> 0.05). In patients with gastric adenocarcinoma, CYP1B1, GSTO1, GSTT1, and GSTK1 protein expressions are higher in tumor than normal gastric tissues.