International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 205-213
OXIDANT AND ANTIOXIDANT STATUS OF PLASMA AND ERYTHROCYTE IN THE BLEOMYCIN-ADMINISTERED RATS: THE PROTECTIVE ROLE OF ERDOSTEINE AND VITAMIN E
FERAH ARMUTÇU1, SADIK SÖĞÜT1, AHMET GÜREL1, LEVENT KART1, ÖMER COŞKUN1
Karaelmas University, Medical Faculty, Department of Biochemistry and Clinical Biochemistry ZONGULDAK
Keywords: bleomycin, lipid peroxidation, antioxidant enzymes, erdosteine, vitamin e
Bleomycin (BLM) toxicity is associated with lipid peroxidation, which is an autocatalytic mechanism leading to oxidative destruction of cellular membranes, and their destruction can lead to the production of toxic, reactive metabolites and cell death. Therefore, we investigated reactive oxygen species production, antioxidant enzyme activities and protective effect of vitamin E and erdosteine in BLM-administrated rats. Thirty-five Sprague-Dawley rats were divided randomly into four groups as untreated control, BLM, BLM+erdosteine and BLM+vitamin E groups. At the end of the treatment, plasma and erythrocytes were obtained and the levels of thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) were measured. Bleomycin administration resulted in the generation of reactive oxygen species in the blood of rats by decreasing the activities of enzymes SOD, CAT and GSH-Px, while increasing the levels of NO and TBARS, an indicative of lipid peroxidation. Erdosteine and vitamin E treatment prevented the increase in the lipid peroxidation. Erdosteine alone significantly prevented the decrease in SOD, CAT and GSH-Px activities in the erythrocyte and plasma. We suggest that erdosteine is more effective on the prevention of BLM-induced hematotoxicity via antioxidant and free radical scavenger properties than vitamin E at the doses used in the present study. However, further studies at different doses of erdosteine are needed to determine most appropriate doses and to clarify the issue.
FERAH ARMUTÇU1, SADIK SÖĞÜT1, AHMET GÜREL1, LEVENT KART1, ÖMER COŞKUN1
Karaelmas University, Medical Faculty, Department of Biochemistry and Clinical Biochemistry ZONGULDAK
Keywords: bleomycin, lipid peroxidation, antioxidant enzymes, erdosteine, vitamin e
Bleomycin (BLM) toxicity is associated with lipid peroxidation, which is an autocatalytic mechanism leading to oxidative destruction of cellular membranes, and their destruction can lead to the production of toxic, reactive metabolites and cell death. Therefore, we investigated reactive oxygen species production, antioxidant enzyme activities and protective effect of vitamin E and erdosteine in BLM-administrated rats. Thirty-five Sprague-Dawley rats were divided randomly into four groups as untreated control, BLM, BLM+erdosteine and BLM+vitamin E groups. At the end of the treatment, plasma and erythrocytes were obtained and the levels of thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) were measured. Bleomycin administration resulted in the generation of reactive oxygen species in the blood of rats by decreasing the activities of enzymes SOD, CAT and GSH-Px, while increasing the levels of NO and TBARS, an indicative of lipid peroxidation. Erdosteine and vitamin E treatment prevented the increase in the lipid peroxidation. Erdosteine alone significantly prevented the decrease in SOD, CAT and GSH-Px activities in the erythrocyte and plasma. We suggest that erdosteine is more effective on the prevention of BLM-induced hematotoxicity via antioxidant and free radical scavenger properties than vitamin E at the doses used in the present study. However, further studies at different doses of erdosteine are needed to determine most appropriate doses and to clarify the issue.